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Tardigrades are microscopic organisms with exceptional resilience to environmental extremes. Most protocols to visualize the internal anatomy of tardigrades rely on fixation, hampering our understanding of dynamic changes to organelles and other subcellular components. Here, we provide protocols for staining live tardigrade adults and other postembryonic stages, facilitating real-time visualization of structures including lipid droplets, mitochondria, lysosomes, and DNA.more » « less
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Heard, Madison; Nordheim, Caitlin; McKinley, Cheyenne; Zilz, Zoe; Jones, Victoria; Vincent, Bridget; Caves, Eleanor (, Oceanography)Undergraduates make up a critical portion of STEM research workers at universities. However, undergraduate researchers coming from racial, cultural, socioeconomic, gender, or other groups that are historically underrepresented in STEM (i.e., excluded identity [EI]; Bhatti, 2021) often have difficulty accessing professional development opportunities that might encourage them to pursue a career in a STEM field (Barber et al., 2020). To support EI students and increase retention in STEM beyond the undergraduate level, opportunities to build scientific skills should have a low barrier to entry and provide ample training.more » « less
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